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KMID : 0624620140470090518
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BMB Reports
2014 Volume.47 No. 9 p.518 ~ p.523
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Capicua is involved in Dorsal-mediated repression of zerkn?llt expression in Drosophila embryo
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Shin Dong-Hyeon
Hong Joung-Woo
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Abstract
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The maternal transcription factor Dorsal (Dl) functions as both an activator and a repressor in a context-dependent manner to control dorsal-ventral patterning in the Drosophila embryo. Previous studies have suggested that Dl is an intrinsic activator and its repressive activity requires additional corepressors that bind corepressor-binding sites near Dl-binding sites. However, the molecular identities of the corepressors have yet to be identified. Here, we present evidence that Capicua (Cic) is involved in Dl-mediated repression in the zerkn?llt (zen) ventral repression element (VRE). Computational and genetic analyses indicate that a DNA-binding consensus sequence of Cic is highly analogous with previously identified corepressorbinding sequences and that Dl failed to repress zen expression in lateral regions of cic mutant embryos. Furthermore, electrophoretic mobility shift assay (EMSA) shows that Cic directly interacts with several corepressor-binding sites in the zen VRE. These results suggest that Cic may function as a corepressor by binding the VRE.
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KEYWORD
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Capicua, Corepressor, Dorsal-mediated repression, Drosophila embryo, VRE, Zerkn?llt
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